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Novak Djokovic's preparations for his Australian Open title defence were dealt a blow with an injury-impacted defeat to Alex De Minaur in the United Cup.

Djokovic lost in two sets to the world number 12 as Australia took a 1-0 lead in their quarter-final against Serbia before they sealed a 2-0 win in the mixed team event.

The world number one twice required treatment from a physio as his right wrist was causing him some problems.

The world number one needed a medical time-out for the issue in his previous United Cup match against the Czech Republic's Jiri Lehecka, which he won in three sets on Tuesday.

The Serbian was treated after holding serve to lead 4-3 in the opening set and again after De Minaur had broken to edge 5-4 up.

Despite Djokovic's fitness concerns, De Minaur produced an impressive performance to seal one of the biggest wins of his career in just over an hour-and-a-half.

De Minaur said in his on-court interview after the match: "It's extra special. Novak is an unbelievable competitor and what he's done for the sport is pretty special.

"When you go up against Novak you have to go out and enjoy it and back yourself and no matter what keep fighting until the end. Today was my day and I'm happy that I was able to get the win."

Ajla Tomljanovic followed De Minaur's win by beating Serbia's Natalija Stevanovic 6-1 6-1 to clinch Australia's semi-final place.